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Fibronectin antibody [2F4]

Anti-Fibronectin antibody [2F4] used in Western Blot (WB). GTX60570
Anti-Fibronectin antibody [2F4] used in Western Blot (WB). GTX60570
Anti-Fibronectin antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60570
Anti-Fibronectin antibody [2F4] used in ELISA (ELISA). GTX60570
Anti-Fibronectin antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60570
Anti-Fibronectin antibody [2F4] used in Flow cytometry (FACS). GTX60570

Cat. No. GTX60570

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Fibronectin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

263 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FN1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887980

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fibronectin antibody [2F4] used in Western Blot (WB). GTX60570

GTX60570 WB Image

WB analysis of human Fibronectin (AA: 1965-2176) recombinant protein using GTX60570 Fibronectin antibody [2F4].

Anti-Fibronectin antibody [2F4] used in Western Blot (WB). GTX60570

GTX60570 WB Image

WB analysis of HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate using GTX60570 Fibronectin antibody [2F4].

Anti-Fibronectin antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60570

GTX60570 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60570 Fibronectin antibody [2F4].

Anti-Fibronectin antibody [2F4] used in ELISA (ELISA). GTX60570

GTX60570 ELISA Image

ELISA analysis of antigen using GTX60570 Fibronectin antibody [2F4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Fibronectin antibody [2F4] used in IHC (Paraffin sections) (IHC-P). GTX60570

GTX60570 IHC-P Image

IHC-P analysis of stomach cancer tissue using GTX60570 Fibronectin antibody [2F4].

Anti-Fibronectin antibody [2F4] used in Flow cytometry (FACS). GTX60570

GTX60570 FACS Image

FACS analysis of HeLa cells using GTX60570 Fibronectin antibody [2F4].
Green : Fibronectin
Red : negative control

REFERENCE

Application Reference

Lee J et al. Invest Ophthalmol Vis Sci 2020; 61 (8) : 41 ZEB1 Mediates Fibrosis in Corneal Endothelial Mesenchymal Transition Through SP1 and SP3.
Application : WB
Reactivity : Human
Lee J et al. J Biol Chem 2018; (Epub) Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399