GeneTex
United States (US)

Fibronectin antibody [3F12]

GTX15737_40_ELISA_19011519_163.jpg
GTX15737_1520_WB_19011519_616.jpg
GTX15737_291_ICC-IF_19011519_776.jpg
GTX15737_1519_WB_19011519_346.jpg

Cat No. GTX15737

Host Mouse
Clonality Monoclonal
Clone Name 3F12
Isotype IgG1
Application WB, ICC/IF, IP, ELISA, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:500
ICC/IF Assay dependent
IP Assay dependent
ELISA 1 μg/ml
RIA Assay dependent
Not tested in other applications.

Calculated MW

263 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 2.5mM KCl, 0.135M NaCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human plasma fibonectin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibronectin 1,Cig,Ed-B,Finc,Fn,Fnz,Gfnd,Gfnd2,Lets,Msf,Smdcf,Fn1

Cellular Localization

Extracellular matrix,Extracellular space,Secreted

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES
GTX15737_40_ELISA_19011519_163.jpg

GTX15737 ELISA Image

Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 µg/mL and fibronectin polyclonal antibody (capture antibody).

GTX15737_1520_WB_19011519_616.jpg

GTX15737 WB Image

WB analysis of 30 µg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 2 µg/ml

GTX15737_291_ICC-IF_19011519_776.jpg

GTX15737 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 overnight at 4 °C

GTX15737_1519_WB_19011519_346.jpg

GTX15737 WB Image

WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 1:300

Package List Price ($)
$ 319