APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:500 |
| Assay dependent |
| Assay dependent |
| 1 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM Potassium Phosphate, 2.5mM KCl, 135mM NaCl, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human plasma fibonectin
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
![Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737](/templates/default/js/lightbox/images/loading.gif "Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737")
|
GTX15737 WB Image
WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 1:300
|
|
|
GTX15737 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 overnight at 4 ºC
|
|
|
GTX15737 WB Image
WB analysis of 30 μg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 2 μg/ml
|
|
|
GTX15737 ELISA Image
Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 μg/mL and fibronectin polyclonal antibody (capture antibody).
|
REFERENCE
There are currently no references for Fibronectin antibody [3F12] (GTX15737). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fibronectin antibody [3F12] (GTX15737). Be the first to share your experience with this product.
![Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737](/upload/website/prouct_img/small/GTX15737/GTX15737_1519_WB_w_23060620_400.webp "Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737")
![Anti-Fibronectin antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15737](/upload/website/prouct_img/small/GTX15737/GTX15737_291_ICC-IF_w_23060620_643.webp "Anti-Fibronectin antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15737")
![Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737](/upload/website/prouct_img/small/GTX15737/GTX15737_1520_WB_w_23060620_590.webp "Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737")
![Anti-Fibronectin antibody [3F12] used in ELISA (ELISA). GTX15737](/upload/website/prouct_img/small/GTX15737/GTX15737_40_ELISA_w_23060620_853.webp "Anti-Fibronectin antibody [3F12] used in ELISA (ELISA). GTX15737")
![Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737](/upload/website/prouct_img/normal/GTX15737/GTX15737_1519_WB_w_23060620_400.webp "Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737")
![Anti-Fibronectin antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15737](/upload/website/prouct_img/normal/GTX15737/GTX15737_291_ICC-IF_w_23060620_643.webp "Anti-Fibronectin antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15737")
![Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737](/upload/website/prouct_img/normal/GTX15737/GTX15737_1520_WB_w_23060620_590.webp "Anti-Fibronectin antibody [3F12] used in Western Blot (WB). GTX15737")
![Anti-Fibronectin antibody [3F12] used in ELISA (ELISA). GTX15737](/upload/website/prouct_img/normal/GTX15737/GTX15737_40_ELISA_w_23060620_853.webp "Anti-Fibronectin antibody [3F12] used in ELISA (ELISA). GTX15737")