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Fibronectin antibody [N1N2], N-term

Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in ELISA (ELISA). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in ELISA (ELISA). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794
Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

Cat No. GTX112794

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Positive Control

HepG2 , HepG2(10 mM Nicotinamide and 0.4 mM Trichostatin A treatment for 48 hr) , HeLa mock and shFN1 , mouse plasma

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibronectin 1,Cig,Ed-B,Finc,Fn,Fnz,Gfnd,Gfnd2,Lets,Msf,Smdcf,Fn1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

GTX112794 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Fibronectin antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112794

GTX112794 IP Image

Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Fibronectin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112794

GTX112794 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.

Anti-Fibronectin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112794

GTX112794 IHC-P Image

Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

GTX112794 WB Image

Fibronectin antibody detects Fibronectin protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody (GTX112794) at a dilution of 1:1000.

Anti-Fibronectin antibody [N1N2], N-term used in ELISA (ELISA). GTX112794

GTX112794 ELISA Image

Indirect ELISA analysis was performed by coating plate with 100 µL of recombinant Fibronectin protein at concentration of 10 µg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.

Anti-Fibronectin antibody [N1N2], N-term used in ELISA (ELISA). GTX112794

GTX112794 ELISA Image

Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 µg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.

Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

GTX112794 WB Image

Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

GTX112794 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169