GeneTex
United States (US)

Fibronectin antibody [N1N2], N-term

Orthogonal
GTX112794_41941_20180817_WB_shRNA_watermark_18121410_873.jpg
GTX112794_40079_IP_18121410_746.jpg
GTX112794_IFA_18121410_186.jpg
GTX112794_40079_IHC_18121410_114.jpg
GTX112794_41941_20141231_WB_18121410_767.jpg
GTX112794_41941_20180608_ELISA_18121410_799.jpg
GTX112794_41941_20180608_ELISA_Competitive_18121410_634.jpg
GTX112794_41633_20151015_WB_M_plasma_18121410_709.jpg
GTX112794_41633_20170112_WB_Nicotinamide_TSA_18121410_982.jpg

Cat No. GTX112794

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Positive Control

HepG2 , HepG2(10 mM Nicotinamide and 0.4 mM Trichostatin A treatment for 48 hr) , HeLa mock and shFN1 , mouse plasma

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CIG antibody, DKFZp686F10164 antibody, DKFZp686H0342 antibody, DKFZp686I1370 antibody, DKFZp686O13149 antibody, ED-B antibody, FINC antibody, FN antibody, FNZ antibody, GFND antibody, GFND2 antibody, LETS antibody, MSF antibody, FN1 antibody, migration-stimulating factor antibody, fibronectin antibody, cold-insoluble globulin antibody, fibronectin 1 antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112794_41941_20180817_WB_shRNA_watermark_18121410_873.jpg

GTX112794 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX112794_40079_IP_18121410_746.jpg

GTX112794 IP Image

Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX112794_IFA_18121410_186.jpg

GTX112794 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.

GTX112794_40079_IHC_18121410_114.jpg

GTX112794 IHC-P Image

Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112794_41941_20141231_WB_18121410_767.jpg

GTX112794 WB Image

Fibronectin antibody detects Fibronectin protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody (GTX112794) at a dilution of 1:1000.

GTX112794_41941_20180608_ELISA_18121410_799.jpg

GTX112794 ELISA Image

Indirect ELISA analysis was performed by coating plate with 100 µL of recombinant Fibronectin protein at concentration of 10 µg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.

GTX112794_41941_20180608_ELISA_Competitive_18121410_634.jpg

GTX112794 ELISA Image

Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 µg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.

GTX112794_41633_20151015_WB_M_plasma_18121410_709.jpg

GTX112794 WB Image

Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112794_41633_20170112_WB_Nicotinamide_TSA_18121410_982.jpg

GTX112794 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169