PRODUCT
Summary
Fibronectin antibody recognizes fibronectin protein, which is an extracellular glycoprotein (predicted molecular weight of 272 kDa) that exists in two forms. Insoluble fibronectin, made by fibroblasts, chondrocytes, endothelial cells, macrophages, and certain epithelial cells, binds to multiple matrix proteins such as collagens, syndecans, and integrins to mediate cell adhesion and migration. Soluble fibronectin, produced by hepatocytes as a component of vertebrate blood plasma, is involved in blood clotting and wound repair.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse plasma , HepG2 , HeLa
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.48 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950298
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF
Cellular Localization
Secreted , extracellular space , extracellular matrix
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112794 IHC-P Image
Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112794 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112794 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX112794 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.
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GTX112794 WB Image
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112794 ELISA Image
Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.
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GTX112794 ELISA Image
Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 μg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.
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GTX112794 IHC-P Image
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112794 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112794 IHC-P Image
Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112794 IP Image
Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX112794 IHC-P Image
The data was published in the journal Oncotarget in 2016.PMID: 26840566
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GTX112794 ICC/IF Image
The data was published in the journal Oncotarget in 2016.PMID: 26840566
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GTX112794 WB Image
The data was published in the journal Nat Commun in 2017.PMID: 28102225
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GTX112794 WB Image
The data was published in the journal Nat Commun in 2017.PMID: 28102225
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GTX112794 WB Image
The data was published in the journal EMBO Rep in 2020. PMID: 31908141
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GTX112794 WB Image
The data was published in the journal EMBO Rep in 2020. PMID: 31908141
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GTX112794 WB Image
The data was published in the journal EMBO Rep in 2020. PMID: 31908141
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GTX112794 WB Image
The data was published in the journal Nat Commun in 2015. PMID: 26245647
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GTX112794 WB Image
The data was published in the journal Nat Commun in 2015. PMID: 26245647
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GTX112794 WB Image
The data was published in the journal Oncotarget in 2015. PMID: 25460509
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REFERENCE
REVIEW
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