Fibronectin antibody [N1N2], N-term

Name: Fibronectin antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX112794
Price: 399.00 USD

Anti-Fibronectin antibody [N1N2], N-term used in Western Blot (WB). GTX112794

Anti-Fibronectin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112794

Anti-Fibronectin antibody [N1N2], N-term used in ELISA (ELISA). GTX112794

Anti-Fibronectin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112794

Anti-Fibronectin antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112794

Cat. No. GTX112794

Reference 61

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX112794 on CiteAb

PRODUCT

Summary

Fibronectin antibody recognizes fibronectin protein, which is an extracellular glycoprotein (predicted molecular weight of 272 kDa) that exists in two forms. Insoluble fibronectin, made by fibroblasts, chondrocytes, endothelial cells, macrophages, and certain epithelial cells, binds to multiple matrix proteins such as collagens, syndecans, and integrins to mediate cell adhesion and migration. Soluble fibronectin, produced by hepatocytes as a component of vertebrate blood plasma, is involved in blood clotting and wound repair.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IP	1:100-1:500
ELISA	1:1000-1:10000

Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Positive Control

mouse plasma , HepG2 , HeLa

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950298

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Gene ID: 2335 FN1
UniProt: P02751 FN1

Research Area

DATA IMAGES

GTX112794 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112794 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX112794 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.

GTX112794 WB Image

Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112794 ELISA Image

Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.

GTX112794 ELISA Image

Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 μg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.

GTX112794 IHC-P Image

Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112794 IP Image

Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX112794 IHC-P Image

Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112794 IHC-P Image

Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112794 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.