APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human FN1(Accession P02751), corresponding to amino acid residues Q2352-E2386._x000D_
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF
Cellular Localization
Secreted, extracellular space, extracellular matrix
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
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GTX04498 ICC/IF Image
ICC/IF analysis of PFA fixed HepG2 cells with Forskolin treatment (4μM, 30min) using GTX04498 Fibronectin antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
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GTX04498 IHC-P Image
IHC-P analysis of mouse ovary tissue using GTX04498 Fibronectin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04498 WB Image
WB analysis of PC12 whole cell lysates using GTX04498 Fibronectin antibody. The lane on the left was treated with blocking peptide.
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GTX04498 WB Image
WB analysis of various sample lysates using GTX04498 Fibronectin antibody.
Lane 1 : Serum starvated HepG2 whole cell lysates with blocking peptide treatment
Lane 2 : Serum starvated HepG2 whole cell lysates
Lane 3 : HeLa whole cell lysates with LPS treatment (4hr)
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GTX04498 WB Image
WB analysis of various sample lysates using GTX04498 Fibronectin antibody.
Lane 1 : MDA whole cell lysates with blocking peptide treatment
Lane 2 : MDA whole cell lysates
Lane 3 : EC-304 whole cell lysates
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REFERENCE
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REVIEW
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