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Fibronectin antibody

Cat No. GTX20299

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA, IHC

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000 - 1:10000
IP 1:100
ELISA 1:5000 - 1:10000
IHC 1:50 - 1:200
Not tested in other applications.

Calculated MW

263 kDa. ( Note )

Product Note

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.  This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin.  Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.? The eluted material was further purified by gel filtration.? Immunization occurred after single-band purity was

Purification

Purified by antigen-affinity chromatography.
Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has bee

Conjugation

Unconjugated

RRID

AB_377934

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF

Background

Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339