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Fibronectin antibody

Cat No. GTX72724

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, IHC

Reactivity

Human, Pig
Package
500 μl ($219)

APPLICATION

Application Note

Immunohistochemistry (IHC) 1:100-1:200 for 30 minute at room temperature using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temperature for 20 minutes.

Calculated MW

263 kDa. ( Note )

Positive Control

Human kidney

PROPERTIES

Form

Liquid

Buffer

10 mM PO4, 150 mM NaCl, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Fibronectin from human plasma.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fibronectin 1 , Cig , Ed-B , Finc , Fn , Fnz , Gfnd , Gfnd2 , Lets , Msf , Smdcf , Fn1

Cellular Localization

Connective tissue matrix and basement membrane.

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf