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Filaggrin antibody [FLG01]

Orthogonal
Anti-Filaggrin antibody [FLG01] used in Western Blot (WB). GTX23137
Anti-Filaggrin antibody [FLG01] used in IHC (Frozen sections) (IHC-Fr). GTX23137
Anti-Filaggrin antibody [FLG01] used in IHC (Frozen sections) (IHC-Fr). GTX23137
Anti-Filaggrin antibody [FLG01] used in Western Blot (WB). GTX23137
Anti-Filaggrin antibody [FLG01] used in (). GTX23137
Anti-Filaggrin antibody [FLG01] used in (). GTX23137
Anti-Filaggrin antibody [FLG01] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23137

Cat. No. GTX23137

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 FLG01

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
500 μl ($399)
See all Filaggrin products

APPLICATION

Application Note

Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4μg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Calculated MW

435 kDa. ( Note )

Positive Control

Skin

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_384952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

filaggrin , ATOD2

Background

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-Filaggrin antibody [FLG01] used in Western Blot (WB). GTX23137

GTX23137 WB Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in IHC (Frozen sections) (IHC-Fr). GTX23137

GTX23137 IHC-Fr Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in IHC (Frozen sections) (IHC-Fr). GTX23137

GTX23137 IHC-Fr Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in Western Blot (WB). GTX23137

GTX23137 WB Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in (). GTX23137

GTX23137 Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in (). GTX23137

GTX23137 Image

Orthogonal

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Anti-Filaggrin antibody [FLG01] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23137

GTX23137 ICC/IF Image

The data was published in the journal Cell Death Dis in 2018. PMID: 29545605

REFERENCE

Application Reference

Oh JS et al. Nutrients 2021; 13 (9) Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice.
Application : WB
Reactivity : Mouse
Gutowska-Owsiak D et al. Cell Death Dis 2018; 9 (4) : 412 Orchestrated control of filaggrin-actin scaffolds underpins cornification.
Application : ICC/IF
Reactivity : Human
Naeem AS et al. J Allergy Clin Immunol 2017; 139 (4) : 1228-1241 A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis.
Kolesnik M et al. Acta Derm Venereol 2017; : Epub ahead of print Eczema in Psoriatico: An Important Differential Diagnosis Between Chronic Allergic Contact Dermatitis and Psoriasis in Palmoplantar Localization.
Application : IHC-P
Reactivity : Human
Biedermann T et al. J Invest Dermatol 2010; 130 (8) : 1996-2009 Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399