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Filaggrin antibody

Anti-Filaggrin antibody used in Western Blot (WB). GTX03270
Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270
Anti-Filaggrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03270
Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270
Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270
Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270
Anti-Filaggrin antibody used in Western Blot (WB). GTX03270

Cat. No. GTX03270

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Filaggrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

435 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Filaggrin, corresponding to a region within C-terminal amino acids.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

filaggrin , ATOD2

Cellular Localization

Cytoplasmic granule

Background

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-Filaggrin antibody used in Western Blot (WB). GTX03270

GTX03270 WB Image

WB analysis of rat eye lysates using GTX03270 Filaggrin antibody. The lane on the left was treated with blocking peptide.

Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270

GTX03270 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX03270 Filaggrin antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Filaggrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03270

GTX03270 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03270 Filaggrin antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270

GTX03270 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX03270 Filaggrin antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270

GTX03270 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX03270 Filaggrin antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Filaggrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03270

GTX03270 IHC-P Image

IHC-P analysis of human colorectal cancer and adjacent normal tissue using GTX03270 Filaggrin antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Filaggrin antibody used in Western Blot (WB). GTX03270

GTX03270 WB Image

WB analysis of HeLa cell lysate using GTX03270 Filaggrin antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399