APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse kidney , H1299 , HepG2 , PC-12 , Rat2
Predict Reactivity
Rabbit, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.42 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Filamin A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036932
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
filamin A , ABP-280 , ABPX , CSBS , CVD1 , FGS2 , FLN , FLN-A , FLN1 , FMD , MNS , NHBP , OPD , OPD1 , OPD2 , XLVD , XMVD
Cellular Localization
Cytoplasm , cell cortex , cytoskeleton
Background
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
Database
Research Area
DATA IMAGES
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GTX112939 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Filamin A alpha 2(GTX112939) antibody at 1:200 dilution.
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GTX112939 WB Image
Filamin A antibody detects Filamin A protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Filamin A antibody (GTX112939) dilution: 1:1000
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GTX112939 WB Image
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 (GTX27900) 5% SDS PAGE GTX112939 diluted at 1:1000
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GTX112939 IHC-P Image
Filamin A antibody [C2], C-term detects Filamin A protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Filamin A antibody [C2], C-term (GTX112939) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112939 WB Image
Sample (50 ug of whole cell lysate) A: mouse kidney 5% SDS PAGE GTX112939 diluted at 1:1000
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REFERENCE
REVIEW
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