APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HeLa , HepG2
Predict Reactivity
Mouse, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888313
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
filamin B , ABP-278 , ABP-280 , AOI , FH1 , FLN-B , FLN1L , LRS1 , SCT , TABP , TAP
Cellular Localization
Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm
Background
This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
![Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425](/templates/default/js/lightbox/images/loading.gif "Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425")
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GTX632425 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT387] (GTX632425) diluted at 1:500.
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GTX632425 ICC/IF Image
Filamin B antibody [GT387] detects Filamin B protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Filamin B protein stained by Filamin B antibody [GT387] (GTX632425) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX632425 IHC-P Image
Filamin B antibody [GT387] detects Filamin B protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Filamin B antibody [GT387] (GTX632425) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632425 WB Image
Filamin B antibody [GT387] detects Filamin B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT387] (GTX632425) diluted at a dilution of 1:1000.
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REFERENCE
There are currently no references for Filamin B antibody [GT387] (GTX632425). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Filamin B antibody [GT387] (GTX632425). Be the first to share your experience with this product.
![Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425](/upload/website/prouct_img/small/GTX632425/GTX632425_42114_20160825_WB_shRNA_watermark_w_23061202_577.webp "Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425")
![Anti-Filamin B antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632425](/upload/website/prouct_img/small/GTX632425/GTX632425_42114_20150924_IFA_w_23061202_443.webp "Anti-Filamin B antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632425")
![Anti-Filamin B antibody [GT387] used in IHC (Paraffin sections) (IHC-P). GTX632425](/upload/website/prouct_img/small/GTX632425/GTX632425_42114_20151026_IHC-P_R_w_23061202_958.webp "Anti-Filamin B antibody [GT387] used in IHC (Paraffin sections) (IHC-P). GTX632425")
![Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425](/upload/website/prouct_img/small/GTX632425/GTX632425_42114_20150605_WB_w_23061202_693.webp "Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425")
![Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425](/upload/website/prouct_img/normal/GTX632425/GTX632425_42114_20160825_WB_shRNA_watermark_w_23061202_577.webp "Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425")
![Anti-Filamin B antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632425](/upload/website/prouct_img/normal/GTX632425/GTX632425_42114_20150924_IFA_w_23061202_443.webp "Anti-Filamin B antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632425")
![Anti-Filamin B antibody [GT387] used in IHC (Paraffin sections) (IHC-P). GTX632425](/upload/website/prouct_img/normal/GTX632425/GTX632425_42114_20151026_IHC-P_R_w_23061202_958.webp "Anti-Filamin B antibody [GT387] used in IHC (Paraffin sections) (IHC-P). GTX632425")
![Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425](/upload/website/prouct_img/normal/GTX632425/GTX632425_42114_20150605_WB_w_23061202_693.webp "Anti-Filamin B antibody [GT387] used in Western Blot (WB). GTX632425")