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Filamin B antibody [N1], N-term

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101206
Anti-Filamin B antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101206

Cat No. GTX101206

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

278 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , NIH-3T3

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

filamin B , ABP-278 , ABP-280 , AOI , FH1 , FLN-B , FLN1L , LRS1 , SCT , TABP , TAP

Cellular Localization

Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm

Background

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody (GTX101206) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
5% SDS-PAGE
Filamin B antibody [N1], N-term (GTX101206) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX101206 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101206

GTX101206 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B(GTX101206) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-Filamin B antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101206

GTX101206 IHC-P Image

Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Filamin B antibody [N1], N-term (GTX101206) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169