GeneTex
United States (US)

Filamin B antibody [N1], N-term

KO/KD
GTX101206_40548_20160825_WB_shRNA_watermark_19011506_206.jpg
GTX101206_40548_20141229_WB_19011506_709.jpg
GTX101206_40548_WB_M_19011506_426.jpg
GTX101206_40548_WB_19011506_662.jpg
GTX101206_39568_IFA_19011506_195.jpg
GTX101206_40548_20151223_IHC-P_19011506_495.jpg

Cat No. GTX101206

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

278 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , NIH-3T3

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Filamin B,Abp-278,Abp-280,Aoi,Fh1,Fln-B,Fln1L,Lrs1,Sct,Tabp,Tap,Flnb

Cellular Localization

Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm

Background

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101206_40548_20160825_WB_shRNA_watermark_19011506_206.jpg

GTX101206 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101206_40548_20141229_WB_19011506_709.jpg

GTX101206 WB Image

Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody (GTX101206) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101206_40548_WB_M_19011506_426.jpg

GTX101206 WB Image

Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell extract
5% SDS-PAGE
Filamin B antibody [N1], N-term (GTX101206) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101206_40548_WB_19011506_662.jpg

GTX101206 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX101206 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101206_39568_IFA_19011506_195.jpg

GTX101206 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B(GTX101206) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX101206_40548_20151223_IHC-P_19011506_495.jpg

GTX101206 IHC-P Image

Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Filamin B antibody [N1], N-term (GTX101206) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169