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Firefly Luciferase antibody

Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125849
Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125849
Anti-Firefly Luciferase antibody used in IHC (Paraffin sections) (IHC-P). GTX125849

Cat. No. GTX125849

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr
Package
100 μl ($399),
25 μl ($169)
See all Firefly Luciferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Firefly Luciferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fire fly Luciferase , Luciferase , Photinus pyralis luciferase , Firefly luciferase

Research Area

DATA IMAGES

Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125849

GTX125849 ICC/IF Image

Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Firefly Luciferase stained by Firefly Luciferase antibody (GTX125849) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125849

GTX125849 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody (GTX125849) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Firefly Luciferase antibody used in IHC (Paraffin sections) (IHC-P). GTX125849

GTX125849 IHC-P Image

The data was published in the journal J Control Release in 2014. PMID: 25449884

REFERENCE

Application Reference

Komatsu H et al. Mol Imaging Biol 2021; 23 (2) : 173-179 Early-Phase Luciferase Signals of Islet Grafts Predicts Successful Subcutaneous Site Transplantation in Rats.
Chen YC et al. Elife 2018; 7 Glucose intake hampers PKA-regulated HSP90 chaperone activity.
Yu-Chen Chen et al. bioRxiv 2018; (Epub) Glucose intake hampers PKA-regulated HSP90 chaperone activity
Application : WB
Srivastava AK et al. Exp Neurol 2016; 289 : 96-102 Serial in vivo imaging of transplanted allogeneic neural stem cell survival in a mouse model of amyotrophic lateral sclerosis.
Application : IHC-Fr
Chehade M et al. Tomography 2016; 2 (2) : 159-165 Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169