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Firefly Luciferase antibody

Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125849
Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125849
Anti-Firefly Luciferase antibody used in IHC (Paraffin sections) (IHC-P). GTX125849

Cat. No. GTX125849

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Firefly Luciferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fire fly Luciferase , Luciferase , Photinus pyralis luciferase , Firefly luciferase

Research Area

DATA IMAGES

Anti-Firefly Luciferase antibody used in Western Blot (WB). GTX125849

GTX125849 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody (GTX125849) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125849

GTX125849 ICC/IF Image

Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Firefly Luciferase stained by Firefly Luciferase antibody (GTX125849) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Firefly Luciferase antibody used in IHC (Paraffin sections) (IHC-P). GTX125849

GTX125849 IHC-P Image

The data was published in the journal J Control Release in 2014. PMID: 25449884

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169