GeneTex
United States (US)

Firefly Luciferase antibody

GTX125849_42809_20170330_WB_B_18121410_280.jpg

Cat No. GTX125849

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

firefly transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Firefly Luciferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fire fly Luciferase, Luciferase, Photinus pyralis luciferase, Firefly luciferase

Research Area

DATA IMAGES
GTX125849_42809_20170330_WB_B_18121410_280.jpg

GTX125849 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody (GTX125849) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169