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Firefly Luciferase antibody

Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20498

Cat. No. GTX20498

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, IHC

Reactivity

 Firefly
Package
500 μg ($399)
See all Firefly Luciferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Firefly

Immunogen

Luciferase [ Photinus pyralis (Firefly)]

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Luciferase is a luminescent enzyme, mostly known for its role as a reporter protein

Research Area

DATA IMAGES

Anti-Firefly Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20498

GTX20498 ICC/IF Image

Figure 1. Immunofluorescence microscopy. NIH3T3 cells transiently transfected with a luciferase gene. Luciferase-positive cells were detected using 20 μg/ml of GeneTex's polyclonal Anti-Luciferase antibody (GTX20498) followed by 1:200 dilution of anti-goat IgG-FITC (green). Cells were mounted using Vectashield with DAPI (blue) and visualized at 200X magnification.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399