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Flavivirus group antibody [D1-4G2-4-15 (4G2)]

Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57154
Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in ELISA (ELISA). GTX57154

Cat No. GTX57154

Host Mouse
Clonality Monoclonal
Clone Name D1-4G2-4-15 (4G2)
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS, ELISA
Package
100 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

WB
Binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions.(Lai et al. 2008)

Not tested in other applications.

Specificity/Sensitivity

Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% Proclin 300.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dengue virus

Immunogen

Dengue Virus type 2 antigens.

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Envelope Protein Antibody, Protein E Antibody

Research Area

DATA IMAGES
Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57154

GTX57154 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed Vero cells infected with ZIKV after 30h infection using GTX57154 Flavivirus group antibody [D1-4G2-4-15 (4G2)]. Staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4°C.
Red : Primary antibody
Blue : DAPI

Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in ELISA (ELISA). GTX57154

GTX57154 ELISA Image

ELISA analysis of Dengue Virus Serotype 2 VLPs (5µg/ml) using serial diluted GTX57154 Flavivirus group antibody [D1-4G2-4-15 (4G2)].

REFERENCE
REVIEW
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