APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , rat muscle , Raji
Predict Reactivity
Zebrafish, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Flightless I. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620802
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
FLII actin remodeling protein , FLI , FLIL , Fli1
Cellular Localization
Nucleus , Cytoplasm , cytoskeleton , Centrosome
Background
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/templates/default/js/lightbox/images/loading.gif "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
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GTX114410 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
Flightless I antibody
GTX114410 diluted at 1:1000
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GTX114410 WB Image
Flightless I antibody [C2C3], C-term detects FLII protein by Western blot analysis.
A. 50 μg rat muscle lysate/extract
5 % SDS-PAGE
Flightless I antibody [C2C3], C-term (GTX114410) dilution: 1:1000
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GTX114410 ICC/IF Image
Flightless I antibody [C2C3], C-term detects FLII protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FLII protein stained by Flightless I antibody [C2C3], C-term (GTX114410) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX114410 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX114410 diluted at 1:1000
|
REFERENCE
There are currently no references for Flightless I antibody [C2C3], C-term (GTX114410). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Flightless I antibody [C2C3], C-term (GTX114410). Be the first to share your experience with this product.
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/small/GTX114410/GTX114410_WB_w_23060501_154.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/small/GTX114410/GTX114410_40184_WB_R_muscle_w_23060501_992.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114410](/upload/website/prouct_img/small/GTX114410/GTX114410_40184_IFA_w_23060501_247.webp "Anti-Flightless I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/small/GTX114410/GTX114410_40184_WB_M_brain_w_23060501_684.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/normal/GTX114410/GTX114410_WB_w_23060501_154.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/normal/GTX114410/GTX114410_40184_WB_R_muscle_w_23060501_992.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114410](/upload/website/prouct_img/normal/GTX114410/GTX114410_40184_IFA_w_23060501_247.webp "Anti-Flightless I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114410")
![Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410](/upload/website/prouct_img/normal/GTX114410/GTX114410_40184_WB_M_brain_w_23060501_684.webp "Anti-Flightless I antibody [C2C3], C-term used in Western Blot (WB). GTX114410")