Flightless I antibody [N3C1], Internal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [N3C1] (GTX106269) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
5% SDS PAGE
GTX106269 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(GTX106269) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.