GeneTex
United States (US)

Flightless I antibody [N3C1], Internal

GTX106269_39946_IFA_mESC_19011506_275.jpg
GTX106269_39946_WB_M_brain_19011506_424.jpg
GTX106269_39946_IFA_19011506_542.jpg
GTX106269_WB_19011506_808.jpg

Cat No. GTX106269

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Flightless I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLI antibody, FLIL antibody, Fli1 antibody, MGC39265 antibody, FLII antibody, protein flightless-1 homolog antibody, flightless I homolog (Drosophila) antibody

Cellular Localization

Nucleus (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Centrosome (By similarity)

Background

This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]

Database

DATA IMAGES
GTX106269_39946_IFA_mESC_19011506_275.jpg

GTX106269 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I(GTX106269) antibody at 1:200 dilution.

GTX106269_39946_WB_M_brain_19011506_424.jpg

GTX106269 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX106269 diluted at 1:1000

GTX106269_39946_IFA_19011506_542.jpg

GTX106269 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(GTX106269) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX106269_WB_19011506_808.jpg

GTX106269 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
5% SDS PAGE
GTX106269 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169