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Flotillin 1 antibody [C3], C-term

Anti-Flotillin 1  antibody [C3], C-term used in Western Blot (WB). GTX104769
Anti-Flotillin 1  antibody [C3], C-term used in Immunoprecipitation (IP). GTX104769

Cat No. GTX104769

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Hamster
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

flotillin 1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein , Melanosome

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Flotillin 1  antibody [C3], C-term used in Western Blot (WB). GTX104769

GTX104769 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Flotillin 1  antibody [C3], C-term used in Immunoprecipitation (IP). GTX104769

GTX104769 IP Image

Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769)
10 % SDS-PAGE
The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
REVIEW

Flotillin 1 antibody [C3], C-term Cat No. GTX104769

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO?DP?12 clone#1933 cells
Amount used : 40μg
Blocking : 3% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169