Flotillin 1 antibody [C3], C-term

Name: Flotillin 1 antibody [C3], C-term
Brand: GeneTex
SKU: GTX104769
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Flotillin 1 antibody [C3], C-term used in Western Blot (WB). GTX104769

Anti-Flotillin 1 antibody [C3], C-term used in Immunoprecipitation (IP). GTX104769

Cat. No. GTX104769

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human, Hamster
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX104769 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240818

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flotillin 1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein , Melanosome

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]

Database

Gene ID: 10211 FLOT1
UniProt: O75955 FLOT1

Research Area

DATA IMAGES

GTX104769 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX104769 IP Image

Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769)
10 % SDS-PAGE
The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX104769 WB Image

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

Hsu XR et al. J Exp Clin Cancer Res 2023; 42 (1) : 283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.

XR Hsu et al. Journal of Experimental & Clinical Cancer Research 2023; 42 : 283 Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer

P?rez-Rodriguez S et al. PLoS One 2020; 15 (8) : e0237930 Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.

Application : WB

Reactivity : Hamster

Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.

Murdica V et al. Hum Reprod 2019; Proteomic analysis reveals the negative modulator of sperm function glycodelin as over-represented in semen exosomes isolated from asthenozoospermic patients.

Application : WB

Reactivity : Human

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