GeneTex
United States (US)

Flotillin 1 antibody [C3], C-term

GTX104769_40205_20181123_WB_19022001_338.jpg
GTX104769_40205_IP_19011506_393.jpg

Cat No. GTX104769

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Flotillin 1,Flot1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein , Melanosome

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104769_40205_20181123_WB_19022001_338.jpg

GTX104769 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX104769_40205_IP_19011506_393.jpg

GTX104769 IP Image

Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769)
10 % SDS-PAGE
The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REVIEW

Flotillin 1 antibody [C3], C-term Cat No. GTX104769

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO?DP?12 clone#1933 cells
Amount used : 40μg
Blocking : 3% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169