APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , mouse brain , rat brain
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240819
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
flotillin 2 , ECS-1 , ECS1 , ESA , ESA1 , M17S1
Cellular Localization
Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein
Background
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX100279 WB Image
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 μg mouse brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100279 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
|
|
GTX100279 WB Image
Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100279 WB Image
Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody (GTX100279) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Observed Flotillin 2 protein is larger than the predicted M.W., possibly due to post-translational modifications. Reference: PLoS One. 2009 Aug 4;4(8):e6497.
|
|
GTX100279 IP Image
Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279). Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
GTX100279 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX100279 WB Image
293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
REFERENCE
There are currently no references for Flotillin 2 antibody [C3], C-term (GTX100279). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Flotillin 2 antibody [C3], C-term (GTX100279). Be the first to share your experience with this product.