GeneTex
United States (US)

Flotillin 2 antibody [C3], C-term

GTX100279_39433_WB_M_brain_19011506_711.jpg
GTX100279_IFA_19011506_797.jpg
GTX100279_39433_WB_R_brain_19011506_311.jpg
GTX100279_39443_WB_19011506_829.jpg
GTX100279_39443_20150625_IP_19011506_553.jpg
GTX100279_IHC_19011506_703.jpg

Cat No. GTX100279

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Flotillin 2,Ecs-1,Ecs1,Esa,Esa1,M17S1,Flot2

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100279_39433_WB_M_brain_19011506_711.jpg

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100279_IFA_19011506_797.jpg

GTX100279 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.

GTX100279_39433_WB_R_brain_19011506_311.jpg

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100279_39443_WB_19011506_829.jpg

GTX100279 WB Image

Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody (GTX100279) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Observed Flotillin 2 protein is larger than the predicted M.W., possibly due to post-translational modifications.
Reference: PLoS One. 2009 Aug 4;4(8):e6497.

GTX100279_39443_20150625_IP_19011506_553.jpg

GTX100279 IP Image

Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279).
Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100279_IHC_19011506_703.jpg

GTX100279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169