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Flotillin 2 antibody [C3], C-term

Orthogonal
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100279
Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

Cat No. GTX100279

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Flotillin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240819

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

flotillin 2 , ECS-1 , ECS1 , ESA , ESA1 , M17S1

Cellular Localization

Cell membrane; Peripheral membrane protein , Membrane , caveola; Peripheral membrane protein

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Flotillin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100279

GTX100279 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10% SDS-PAGE
Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody (GTX100279) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Observed Flotillin 2 protein is larger than the predicted M.W., possibly due to post-translational modifications.
Reference: PLoS One. 2009 Aug 4;4(8):e6497.

Anti-Flotillin 2 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100279

GTX100279 IP Image

Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279).
Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Flotillin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100279

GTX100279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Flotillin 2 antibody [C3], C-term used in Western Blot (WB). GTX100279

GTX100279 WB Image

Orthogonal

293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169