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Frataxin antibody, Internal

Anti-Frataxin antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81888
Anti-Frataxin antibody, Internal used in Western Blot (WB). GTX81888
Anti-Frataxin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81888

Cat No. GTX81888

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
400 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 72-102 amino acids from the Central region of human FXN.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

frataxin , CyaY , FA , FARR , FRDA , X25

Cellular Localization

Mitochondrion,Cytoplasm, cytosol

Background

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-Frataxin antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81888

GTX81888 ICC/IF Image

ICC/IF analysis of WiDr cells using GTX81888 Frataxin antibody, Internal.
Green : Frataxin
Blue : DAPI

Anti-Frataxin antibody, Internal used in Western Blot (WB). GTX81888

GTX81888 WB Image

WB analysis of CEM cell lysate (35ug/lane) using GTX81888 Frataxin antibody, Internal.

Anti-Frataxin antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81888

GTX81888 IHC-P Image

IHC-P analysis of human colon carcinoma using GTX81888 Frataxin antibody, Internal.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf