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Frizzled 10 antibody

Anti-Frizzled 10 antibody used in Western Blot (WB). GTX100358
Anti-Frizzled 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX100358
Anti-Frizzled 10 antibody used in Western Blot (WB). GTX100358

Cat. No. GTX100358

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Frizzled 10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Frizzled 10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240838

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 10 , CD350 , FZ-10 , Fz10 , FzE7 , hFz10

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Frizzled 10 antibody used in Western Blot (WB). GTX100358

GTX100358 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 10 antibody (GTX100358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Frizzled 10 antibody used in IHC (Paraffin sections) (IHC-P). GTX100358

GTX100358 IHC-P Image

Frizzled 10 antibody detects Frizzled 10 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Frizzled 10 stained by Frizzled 10 antibody (GTX100358) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Frizzled 10 antibody used in Western Blot (WB). GTX100358

GTX100358 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 10 antibody (GTX100358) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Mine M et al. PLoS One 2015; 10 (7) : e0131350 miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.
Application : WB
Reactivity : Human
Chen CC et al. Am J Respir Crit Care Med 2014; 190 (4) : 433-44 Shisa3 Is Associated with Prolonged Survival through Promoting β-Catenin Degradation in Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169