Frizzled 2 antibody [HL5743]

Unboiled SK-OV-3 cytosol and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 2 antibody [HL5743] (GTX646694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. (Cyto:cytosol; ME: membrane extract)