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Frizzled 2 antibody, Internal

Anti-Frizzled 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88690
Anti-Frizzled 2 antibody, Internal used in Western Blot (WB). GTX88690
Anti-Frizzled 2 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88690
Anti-Frizzled 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88690

Cat. No. GTX88690

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Frizzled 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
ICC/IF 10μg/ml
IHC-P 2.5μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.

IHC-P
Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles.
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88690-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723342

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 2 , Fz2 , OMOD2 , fz-2 , fzE2 , hFz2

Cellular Localization

Membrane; Multi-pass membrane protein , Cell membrane

Background

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-Frizzled 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88690

GTX88690 IHC-P Image

IHC-P analysis of human liver using GTX88690 Frizzled 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Frizzled 2 antibody, Internal used in Western Blot (WB). GTX88690

GTX88690 WB Image

WB analysis of (A)human heart (0.03μg/ml) and (B)placenta (0.1ug/ml) lysate using GTX88690 Frizzled 2 antibody, Internal.
Loading : 35μg protein in RIPA buffer

Anti-Frizzled 2 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88690

GTX88690 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX88690 Frizzled 2 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Frizzled 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88690

GTX88690 IHC-P Image

IHC-P analysis of human thyroid Gland using GTX88690 Frizzled 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

Application Reference

Luo M et al. Biochem Biophys Res Commun 2019; 513 (2) : 528-533 ALPL regulates the aggressive potential of high grade serous ovarian cancer cells via a non-canonical WNT pathway.
Application : WB
Reactivity : Human
Mine M et al. PLoS One 2015; 10 (7) : e0131350 miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399