GeneTex
United States (US)

Frizzled 2 antibody, Internal

GTX88690_845_IHC-P_18122102_305.jpg
GTX88690_114_ICC-IF_18122102_509.jpg
GTX88690_2101_WB_18122102_989.jpg
GTX88690_846_IHC-P_18122102_327.jpg

Cat No. GTX88690

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
ICC/IF 10μg/ml
IHC-P 2.5μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.

IHC-P
Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles.

Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88690-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Frizzled Class Receptor 2,Fz2,Fz-2,Fze2,Hfz2,Fzd2

Background

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES
GTX88690_845_IHC-P_18122102_305.jpg

GTX88690 IHC-P Image

IHC-P analysis of human liver using GTX88690 Frizzled 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

GTX88690_114_ICC-IF_18122102_509.jpg

GTX88690 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX88690 Frizzled 2 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX88690_2101_WB_18122102_989.jpg

GTX88690 WB Image

WB analysis of (A)human heart (0.03µg/ml) and (B)placenta (0.1ug/ml) lysate using GTX88690 Frizzled 2 antibody, Internal.
Loading : 35µg protein in RIPA buffer

GTX88690_846_IHC-P_18122102_327.jpg

GTX88690 IHC-P Image

IHC-P analysis of human thyroid Gland using GTX88690 Frizzled 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

REFERENCE
Package List Price ($)
$ 329