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Frizzled 4 antibody, Internal

Anti-Frizzled 4 antibody, Internal used in Western Blot (WB). GTX88836

Cat. No. GTX88836

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Frizzled 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88836-PEP).

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DEEERRCDPIRIS, from the internal region of the protein sequence according to NP_036325.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10719552

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 4 , CD344 , EVR1 , FEVR , FZD4S , Fz-4 , Fz4 , FzE4 , GPCR , hFz4

Cellular Localization

Membrane; Multi-pass membrane protein , Cell membrane

Background

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Frizzled 4 antibody, Internal used in Western Blot (WB). GTX88836

GTX88836 WB Image

WB analysis of small intestine (Ileum) cell lysate using GTX88836 Frizzled 4 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Chen CC et al. Am J Respir Crit Care Med 2014; 190 (4) : 433-44 Shisa3 Is Associated with Prolonged Survival through Promoting β-Catenin Degradation in Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399