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Frizzled 6 antibody

Anti-Frizzled 6 antibody used in Western Blot (WB). GTX129938
Anti-Frizzled 6 antibody used in Western Blot (WB). GTX129938

Cat No. GTX129938

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929 , 293T , 293T membrane extract

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Frizzled 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

frizzled class receptor 6 , FZ-6 , FZ6 , HFZ6 , NDNC10

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD6 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and 7 transmembrane domains. However, unlike many other Fz family members, FDZ6 does not contain a C-terminal PDZ domain-binding motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Frizzled 6 antibody used in Western Blot (WB). GTX129938

GTX129938 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 6 antibody (GTX129938) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Frizzled 6 antibody used in Western Blot (WB). GTX129938

GTX129938 WB Image

Various cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 6 antibody (GTX129938) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169