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Frizzled 7 antibody, C-term

Anti-Frizzled 7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88831
Anti-Frizzled 7 antibody, C-term used in Western Blot (WB). GTX88831

Cat. No. GTX88831

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Frizzled 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88831-PEP).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RFYHRLSHSSKGET, from the C Terminus of the protein sequence according to NP_003498.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725748

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 7 , FzE3

Cellular Localization

Cell membrane , Endosome membrane

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Frizzled 7 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88831

GTX88831 IHC-P Image

IHC-P analysis of human spleen using GTX88831 Frizzled 7 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-Frizzled 7 antibody, C-term used in Western Blot (WB). GTX88831

GTX88831 WB Image

WB analysis of HepG2 cell lysate using GTX88831 Frizzled 7 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Chen CC et al. Am J Respir Crit Care Med 2014; 190 (4) : 433-44 Shisa3 Is Associated with Prolonged Survival through Promoting β-Catenin Degradation in Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399