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Frizzled 9 antibody [HL1675]

Recombinant antibody KO/KD
Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274
Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274
Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274

Cat. No. GTX637274

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1675

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Frizzled 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Product Note

This antibody was raised against human Frizzled 9 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Frizzled 9. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 9 , CD349 , FZD3

Cellular Localization

Cell membrane

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274

GTX637274 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [HL1675] (GTX637274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274

GTX637274 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [HL1675] (GTX637274) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Frizzled 9 antibody [HL1675] used in Western Blot (WB). GTX637274

GTX637274 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [HL1675] (GTX637274) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations