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Frizzled 9 antibody [N1N2], N-term

Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169
Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169
Anti-Frizzled 9 antibody [N1N2], N-term used in Western Blot (WB). GTX100169

Cat No. GTX100169

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

U87-MG

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Frizzled 9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

frizzled class receptor 9 , CD349 , FZD3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169

GTX100169 IHC-P Image

Frizzled 9 antibody [N1N2], N-term detects Frizzled 9 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Frizzled 9 antibody [N1N2], N-term (GTX100169) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169

GTX100169 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U374 xenograft, using Frizzled 9(GTX100169) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Frizzled 9 antibody [N1N2], N-term used in Western Blot (WB). GTX100169

GTX100169 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [N1N2], N-term (GTX100169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169