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Frizzled 9 antibody [N1N2], N-term

Anti-Frizzled 9 antibody [N1N2], N-term used in Western Blot (WB). GTX100169
Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169
Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169

Cat. No. GTX100169

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Frizzled 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Frizzled 9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

frizzled class receptor 9 , CD349 , FZD3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Frizzled 9 antibody [N1N2], N-term used in Western Blot (WB). GTX100169

GTX100169 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [N1N2], N-term (GTX100169) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169

GTX100169 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U374 xenograft, using Frizzled 9(GTX100169) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Frizzled 9 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100169

GTX100169 IHC-P Image

Frizzled 9 antibody [N1N2], N-term detects Frizzled 9 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Frizzled 9 antibody [N1N2], N-term (GTX100169) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Mine M et al. PLoS One 2015; 10 (7) : e0131350 miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169