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Fukutin antibody [N3C3-2]

Anti-Fukutin antibody [N3C3-2] used in Western Blot (WB). GTX110302
Anti-Fukutin antibody [N3C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110302

Cat. No. GTX110302

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Fukutin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

H1299 , HeLa

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fukutin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950288

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fukutin , CMD1X , FCMD , LGMD2M , LGMDR13 , MDDGA4 , MDDGB4 , MDDGC4

Background

The protein encoded by this gene is a putative transmembrane protein that is localized to the cis-Golgi compartment, where it may be involved in the glycosylation of alpha-dystroglycan in skeletal muscle. The encoded protein is thought to be a glycosyltransferase and could play a role in brain development. Defects in this gene are a cause of Fukuyama-type congenital muscular dystrophy (FCMD), Walker-Warburg syndrome (WWS), limb-girdle muscular dystrophy type 2M (LGMD2M), and dilated cardiomyopathy type 1X (CMD1X). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fukutin antibody [N3C3-2] used in Western Blot (WB). GTX110302

GTX110302 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX110302 diluted at 1:1000

Anti-Fukutin antibody [N3C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110302

GTX110302 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Fukutin(GTX110302) antibody at 1:200 dilution.

REFERENCE

Application Reference

Tsukui R et al. Neuropathology 2022; (Epub) Fukutin regulates tau phosphorylation and synaptic function: Novel properties of fukutin in neurons.
Application : IHC-P
Reactivity : Human
Okamura Y et al. Int J Mol Sci 2021; 22 (22) Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169