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Fumarate hydratase ELISA Pair

Fumarate hydratase ELISA Pair. GTX500033

Cat No. GTX500033

Application

ELISA, Sandwich ELISA

Reactivity

Human
Package
1 pair ($399)
PRODUCT

Product Content

GTX110128
Type : Capture Antibody
Host : Rabbit
Conjugation : Unconjugated
Package : 50 μl
GTX89022
Type : Detection Antibody
Host : Goat
Conjugation : Unconjugated
Package : 50 μl

APPLICATION

Application Note

We recommend using the capture antibody at a concentration of 2μg/mL and the detection antibody at a concentration of 1.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC.  For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fumarate Hydratase , Fmrd , Hlrcc , Lrcc , Mcl , Mcul1 , Fh

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Research Area

DATA IMAGES

Fumarate hydratase ELISA Pair. GTX500033

GTX500033 ELISA Image

ELISA detection of Fumarate hydratase using GTX110128 for capture at a concentration of 2 μg/mL and GTX89022 for detection at a concentration of 1.5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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