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Fumarate hydratase antibody [GT548]

KO/KD
Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145
Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145
Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145

Cat. No. GTX630145

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT548

Isotype

 IgG2b

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fumarate hydratase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

rat liver , mouse liver , 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888186

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1

Cellular Localization

Mitochondrion , Cytoplasm

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145

GTX630145 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT548] (GTX630145) diluted at 1:500.

Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145

GTX630145 WB Image

Fumarate hydratase antibody [GT548] detects Fumarate hydratase protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody [GT548] (GTX630145) dilution: 1:500

Anti-Fumarate hydratase antibody [GT548] used in Western Blot (WB). GTX630145

GTX630145 WB Image

Fumarate hydratase antibody [GT548] detects Fumarate hydratase protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
10 % SDS-PAGE
Fumarate hydratase antibody [GT548] (GTX630145) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations