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Fumarate hydratase antibody [N2C2], Internal

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128
Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128

Cat No. GTX110128

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

mouse liver , 293T , A431 , rat liver

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950282

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1

Cellular Localization

Mitochondrion , Cytoplasm

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein by western blot analysis.
A.50 μg rat liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Fumarate hydratase antibody [N2C2], Internal detects FH protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in Western Blot (WB). GTX110128

GTX110128 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX110128 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110128

GTX110128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (GTX110128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fumarate hydratase antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110128

GTX110128 ICC/IF Image

Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169