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Fumarate hydratase antibody

KO/KD
Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877
Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877
Anti-Fumarate hydratase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109877
Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877
Anti-Fumarate hydratase antibody used in IHC (Paraffin sections) (IHC-P). GTX109877
Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877

Cat. No. GTX109877

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fumarate hydratase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

mouse liver , rat liver , 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fumarate hydratase , FMRD , HLRCC , LRCC , MCL , MCUL1

Cellular Localization

Mitochondrion , Cytoplasm

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877

GTX109877 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877

GTX109877 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109877

GTX109877 ICC/IF Image

Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fumarate hydratase stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877

GTX109877 WB Image

Fumarate hydratase antibody detects FH protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5% SDS-PAGE
Fumarate hydratase antibody (GTX109877) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fumarate hydratase antibody used in IHC (Paraffin sections) (IHC-P). GTX109877

GTX109877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (GTX109877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fumarate hydratase antibody used in Western Blot (WB). GTX109877

GTX109877 WB Image

Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis.
A.50 μg rat liver lysate/extract
10% SDS-PAGE
Fumarate hydratase antibody (GTX109877) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen Y et al. Elife 2024; 12 Retinal metabolism displays evidence for uncoupling of glycolysis and oxidative phosphorylation via Cori-, Cahill-, and mini-Krebs-cycle.
Miettinen M et al. Am J Surg Pathol 2016; 40 (12) : 1661-1669 Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.
Application : IHC-P
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169