ICC/IF analysis of 3T3 cells using GTX23467 Furin antibody.

WB analysis of HeLa (Lane 1), HeLa Cas9 (Lane 2), and HeLa Cas9 cells transduced with Furin Lentiviral sgRNA (Lane 3) cell lysates using GTX23467 Furin antibody(Fig. a). A loss of signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that the antibody is specific to Furin(Fig. b).
Dilution : 1:1000
Loading : 30μg

WB analysis of various sample lysates using GTX23467 Furin antibody. Expression of Furin was observed in HEK-293, HaCaT, BeWo, SK-O-V3, Mouse Liver and Rat Liver but not in Daudi and Ramos which are known to be low expressing cell lines (DOI: 10.1099/0022-1317-75-10-2821).
Dilution : 1:1000
Loading : 30μg

WB analysis of various sample lysates using GTX23467 Furin antibody.
Dilution : 1:500
Loading : 30μg

ICC/IF analysis of methanol-fixed HeLa cells using GTX23467 Furin antibody. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

ICC/IF analysis of 4% paraformaldehyde-fixed BeWo cells using GTX23467 Furin antibody. Panel d represents the merged image showing predominantly Golgi Complex and cytoplasmic localization. Panel e represents Daudi cells with lower expression of Furin (DOI: 10.1099/0022-1317-75-10-2821). Panel f represents control cells with no primary antibody to assess the background.
Permeabilization : 0.1% Triton™ X-100 for 15 minutes
Dilution : 2 μg/mL in 0.1% BSA, incubated at 4ºCovernight