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Furin antibody

Anti-Furin antibody used in Western Blot (WB). GTX55628
Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628
Anti-Furin antibody used in IHC (Paraffin sections) (IHC-P). GTX55628
Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628
Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628

Cat No. GTX55628

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-370 of human FURIN (NP_002560.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

furin, paired basic amino acid cleaving enzyme , FUR , PACE , PCSK3 , SPC1

Cellular Localization

Cell membrane,Secreted,Endosome membrane

Background

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-Furin antibody used in Western Blot (WB). GTX55628

GTX55628 WB Image

WB analysis of various sample lysates using GTX55628 Furin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628

GTX55628 ICC/IF Image

ICC/IF analysis of A549 cells using GTX55628 Furin antibody.
Blue : DAPI
Dilution : 1:150

Anti-Furin antibody used in IHC (Paraffin sections) (IHC-P). GTX55628

GTX55628 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX55628 Furin antibody.
Dilution : 1:100

Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628

GTX55628 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55628 Furin antibody.
Blue : DAPI
Dilution : 1:150

Anti-Furin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55628

GTX55628 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55628 Furin antibody.
Blue : DAPI
Dilution : 1:150

REFERENCE

There are currently no references for Furin antibody (GTX55628). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339