U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with G-CSF antibody [GT21] (GTX641067) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below.

Capture: G-CSF antibody [GT21] (GTX641067) (5 μg/mL)
Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 μg/mL)
Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.