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G-CSF antibody [SPM468]

Anti-G-CSF antibody [SPM468] used in IHC (Paraffin sections) (IHC-P). GTX34755

Cat. No. GTX34755

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM468

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey, Macaque
Package
100 μg ($529)
See all G-CSF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C17orf33 , CSF3 , CSF3OS , GCSF , colony stimulating factor 3 , G-CSF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-G-CSF antibody [SPM468] used in IHC (Paraffin sections) (IHC-P). GTX34755

GTX34755 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34755 G-CSF antibody [SPM468].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529