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G-CSF antibody

Anti-G-CSF antibody used in IHC (Paraffin sections) (IHC-P). GTX31157
Anti-G-CSF antibody used in IHC (Paraffin sections) (IHC-P). GTX31157

Cat No. GTX31157

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA, Neutralizing/Inhibition

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 μg/mL. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 31 and 207 of human G-CSF.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Colony Stimulating Factor 3 , C17Orf33 , Csf3Os , Gcsf , Csf3

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-G-CSF antibody used in IHC (Paraffin sections) (IHC-P). GTX31157

GTX31157 IHC-P Image

IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

Anti-G-CSF antibody used in IHC (Paraffin sections) (IHC-P). GTX31157

GTX31157 IHC-P Image

IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 319