GeneTex
United States (US)

G-CSF antibody

GTX31157_IHC-P_1_18121410_627.jpg
GTX31157_IHC-P_18121410_444.jpg

Cat No. GTX31157

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 μg/mL. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

22 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 31 and 207 of human G-CSF.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Colony Stimulating Factor 3,C17Orf33,Csf3Os,Gcsf,Csf3

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

DATA IMAGES
GTX31157_IHC-P_1_18121410_627.jpg

GTX31157 IHC-P Image

IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

GTX31157_IHC-P_18121410_444.jpg

GTX31157 IHC-P Image

IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

Package List Price ($)
$ 319