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G protein alpha Inhibitor 3 antibody [N2C3]

Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676
Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676
Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103676
Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676

Cat No. GTX103676

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Guinea pig, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G protein subunit alpha i3 , 87U6 , ARCND1

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels.

Database

Research Area

DATA IMAGES
Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Wild-type (WT) and G protein alpha Inhibitor 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with G protein alpha Inhibitor 3 antibody [N2C3] (GTX103676) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Sample (30μg whole cell lysate)
A:A431(GTX27909)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
7.5% SDS PAGE
GTX103676 diluted at 1:1000

Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103676

GTX103676 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using G protein alpha Inhibitor 3(GTX103676) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-G protein alpha Inhibitor 3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103676 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169