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G3BP1 antibody [HL2254]

Recombinant antibody Orthogonal
Anti-G3BP1 antibody [HL2254] used in IHC (Paraffin sections) (IHC-P). GTX638303
Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303
Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303
Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303
Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303
Anti-G3BP1 antibody [HL2254] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638303

Cat. No. GTX638303

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2254

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all G3BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human G3BP1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G3BP stress granule assembly factor 1 , G3BP , HDH-VIII

Cellular Localization

Cytoplasm, cytosol , Nucleus

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-G3BP1 antibody [HL2254] used in IHC (Paraffin sections) (IHC-P). GTX638303

GTX638303 IHC-P Image

G3BP1 antibody [HL2254] detects G3BP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
G3BP1 stained by G3BP1 antibody [HL2254] (GTX638303) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303

GTX638303 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (GTX638303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303

GTX638303 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (GTX638303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303

GTX638303 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (GTX638303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G3BP1 antibody [HL2254] used in Western Blot (WB). GTX638303

GTX638303 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (GTX638303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G3BP1 antibody [HL2254] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638303

GTX638303 ICC/IF Image

Orthogonal

G3BP1 antibody [HL2254] detects G3BP1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G3BP1 stained by G3BP1 antibody [HL2254] (GTX638303) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations