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G3BP1 (phospho Ser232) antibody

Anti-G3BP1 (phospho Ser232) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50149
Anti-G3BP1 (phospho Ser232) antibody used in Western Blot (WB). GTX50149
Anti-G3BP1 (phospho Ser232) antibody used in IHC (Paraffin sections) (IHC-P). GTX50149

Cat No. GTX50149

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from human G3BP1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G3BP stress granule assembly factor 1 , G3BP , HDH-VIII

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-G3BP1 (phospho Ser232) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50149

GTX50149 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50149 G3BP1 (phospho Ser232) antibody.

Anti-G3BP1 (phospho Ser232) antibody used in Western Blot (WB). GTX50149

GTX50149 WB Image

WB analysis of extracts from 293 cells untreated or treated with starvation using GTX50149 G3BP1 (phospho Ser232) antibody.

Anti-G3BP1 (phospho Ser232) antibody used in IHC (Paraffin sections) (IHC-P). GTX50149

GTX50149 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50149 G3BP1 (phospho Ser232) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

There are currently no reviews for G3BP1 (phospho Ser232) antibody (GTX50149). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319