APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| 2-4μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
IHC-P
Human Testis shows strong cytoplasm staining in spermatogonia.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89057-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 ; NP_001035810.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glucose-6-phosphate dehydrogenase , G6PD1
Background
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89057 WB Image
WB analysis of human testis lysate using GTX89057 G6PD (aa 308 - 320) antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89057 ELISA Image
ELISA analysis of G6PD protein using GTX89057 G6PD (aa 308 - 320) antibody, Internal.
Capture : GTX113186 (5μg/ml)
Detection : GTX89057 (5μg/ml)
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GTX89057 IHC-P Image
IHC-P analysis of human testis using GTX89057 G6PD (aa 308 - 320) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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REFERENCE
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REVIEW
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