G6PD antibody [N2C1], Internal

Cat. No. GTX113186

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all G6PD products

Datasheet
Document

View product citations for antibody GTX113186 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX113186 , Detection: GTX89057

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950321

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate dehydrogenase , G6PD1

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 2539 G6PD
UniProt: P11413 G6PD

Research Area

DATA IMAGES

GTX113186 IHC-P Image

G6PD antibody [N2C1], Internal detects G6PD protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: G6PD antibody [N2C1], Internal (GTX113186) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113186 ELISA Image

ELISA detection of G6PD using GTX113186 for capture at a concentration of 5 μg/mL and GTX89057 for detection at a concentration of 5 μg/mL.

GTX113186 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX113186 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty