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G6PD antibody [N3C3]

Anti-G6PD antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101218
Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218
Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218
Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

Cat. No. GTX101218

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all G6PD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HeLaS3 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rabbit, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate dehydrogenase , G6PD1

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-G6PD antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101218

GTX101218 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD(GTX101218) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody (GTX101218) diluted at 1:500.

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa S3 (GTX14654)
7.5% SDS PAGE
GTX101218 diluted at 1:500

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody (GTX101218) diluted at 1:500.

REFERENCE

Application Reference

Wang X et al. Cancer Cell Int 2024; 24 (1) : 12 RORα inhibits gastric cancer proliferation through attenuating G6PD and PFKFB3 induced glycolytic activity.
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Silic-Benussi M et al. Redox Biol 2022; 51 : 102268 mTOR inhibition downregulates glucose-6-phosphate dehydrogenase and induces ROS-dependent death in T-cell acute lymphoblastic leukemia cells.
Application : IHC-Fr
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169