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G6PD antibody [N3C3]

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218
Anti-G6PD antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101218
Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218
Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

Cat No. GTX101218

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rabbit, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glucose-6-phosphate dehydrogenase , G6PD1

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody (GTX101218) diluted at 1:500.

Anti-G6PD antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101218

GTX101218 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD(GTX101218) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with G6PD antibody (GTX101218) diluted at 1:500.

Anti-G6PD antibody [N3C3] used in Western Blot (WB). GTX101218

GTX101218 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa S3 (GTX14654)
7.5% SDS PAGE
GTX101218 diluted at 1:500

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169