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G6PD antibody

Orthogonal
Anti-G6PD antibody used in IHC (Paraffin sections) (IHC-P). GTX101212
Anti-G6PD antibody used in Western Blot (WB). GTX101212

Cat. No. GTX101212

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all G6PD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1950320

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate dehydrogenase , G6PD1

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-G6PD antibody used in IHC (Paraffin sections) (IHC-P). GTX101212

GTX101212 IHC-P Image

G6PD antibody detects G6PD protein at cytosol on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
G6PD antibody (GTX101212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G6PD antibody used in Western Blot (WB). GTX101212

GTX101212 WB Image

Orthogonal

The data was published in the journal Aging Cell in 2014. PMID: 24757718

REFERENCE

Application Reference

Anderton B et al. EMBO Rep 2017; 18 (4) : 569-585 MYC-driven inhibition of the glutamate-cysteine ligase promotes glutathione depletion in liver cancer.
Li B et al. Aging Cell 2014; 13 (2) : 367-78 Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells.
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Lu CH et al. Arch Biochem Biophys 2014; 13 (2) : 367-378 Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169