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G9a antibody

Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129153
Anti-G9a antibody used in Immunoprecipitation (IP). GTX129153
Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in ChIP assay (ChIP assay). GTX129153
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX129153

Cat No. GTX129153

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

euchromatic histone lysine methyltransferase 2 , BAT8 , C6orf30 , G9A , GAT8 , KMT1C , NG36

Cellular Localization

Nucleus

Background

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:2000.

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:3000.

Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129153

GTX129153 ICC/IF Image

G9a antibody detects G9a protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G9a protein stained by G9a antibody (GTX129153) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-G9a antibody used in Immunoprecipitation (IP). GTX129153

GTX129153 IP Image

G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 μg of G9a antibody (GTX129153) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody (GTX129153) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

G9a antibody detects G9a protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract |
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
G9a antibody (GTX129153) dilution: 1:1000

Anti-G9a antibody used in ChIP assay (ChIP assay). GTX129153

GTX129153 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.
ChIP experiment and primer designs are based on PLoS Genet. 2014 May 1;10(5):e1004318.

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX129153

GTX129153 IHC-P Image

G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
G9a antibody (GTX129153) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169