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G9a antibody

KO/KD
Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129153
Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX129153
Anti-G9a antibody used in ChIP assay (ChIP assay). GTX129153
Anti-G9a antibody used in Western Blot (WB). GTX129153
Anti-G9a antibody used in Immunoprecipitation (IP). GTX129153

Cat. No. GTX129153

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all G9a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885911

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

euchromatic histone lysine methyltransferase 2 , BAT8 , C6orf30 , G9A , GAT8 , KMT1C , NG36

Cellular Localization

Nucleus

Background

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:3000.

Anti-G9a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129153

GTX129153 ICC/IF Image

G9a antibody detects G9a protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: G9a protein stained by G9a antibody (GTX129153) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-G9a antibody used in IHC (Paraffin sections) (IHC-P). GTX129153

GTX129153 IHC-P Image

G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
G9a antibody (GTX129153) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-G9a antibody used in ChIP assay (ChIP assay). GTX129153

GTX129153 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.
ChIP experiment and primer designs are based on PLoS Genet. 2014 May 1;10(5):e1004318.

Anti-G9a antibody used in Western Blot (WB). GTX129153

GTX129153 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-G9a antibody used in Immunoprecipitation (IP). GTX129153

GTX129153 IP Image

G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 μg of G9a antibody (GTX129153) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody (GTX129153) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1) : 182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : ChIP assay
Reactivity : Rat
Lin HY et al. Biomed Pharmacother 2022; 145 : 112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Zhang K et al. Mol Cancer 2018; 17 (1) : 153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Tian YF et al. Am J Cancer Res 2018; 8 (9) : 1812-1822 Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Application : WB
Reactivity : Human
Luo CW et al. Radiother Oncol 2017; (Epub) G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169