GeneTex
United States (US)

GAB1 antibody

GTX111253_40863_WB_19011507_489.jpg
GTX111253_40863_IHC_19011507_542.jpg
GTX111253_40863_WB_M_19011507_118.jpg

Cat No. GTX111253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HCT116 , MCF-7 , JC , BCL-1 , *C2C12

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Grb2 Associated Binding Protein 1,Dfnb26,Gab1

Background

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111253_40863_WB_19011507_489.jpg

GTX111253 WB Image

Sample (30 µg of whole cell lysate)
A: HCT116
B: MCF-7
7.5% SDS PAGE
GTX111253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111253_40863_IHC_19011507_542.jpg

GTX111253 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GAB1(GTX111253) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111253_40863_WB_M_19011507_118.jpg

GTX111253 WB Image

Sample (30 µg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX111253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

GAB1 antibody Cat No. GTX111253

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 02-Dec-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 20μg
Blocking : 5% skim milk in 0.05% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169