Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Specific for the ~51k alpha 2-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 2-subunit knockout animals.
Form
Liquid
Buffer
10mM HEPES pH7.5, 0.15M NaCl, 0.1mg/ml BSA, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Fusion protein from the cytoplasmic loop of the alpha 2 subunit of rat GABA A receptor.
Purification
Purified by ion exchange chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
gamma-aminobutyric acid type A receptor alpha2 subunit
Cellular Localization
Cell membrane,Cell projection, dendrite
Background
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area