GeneTex
United States (US)

GABA A Receptor alpha 4 antibody, Internal

GTX31003_2273_WB_18121410_590.jpg
GTX31003_937_IHC-P_18121410_381.jpg

Cat No. GTX31003

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Cortex shows patchy staining near or at the neuronal cell membrane..

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX31003-PEP).

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKAKRKTSKPPQE, from the internal region of the protein sequence according to NP_000800.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gamma-Aminobutyric Acid Type A Receptor Alpha4 Subunit,Gabra4

Background

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX31003_2273_WB_18121410_590.jpg

GTX31003 WB Image

WB analysis of human frontal cortex lysate using GTX31003 GABA A Receptor alpha 4 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX31003_937_IHC-P_18121410_381.jpg

GTX31003 IHC-P Image

IHC-P analysis of human cortex using GTX31003 GABA A Receptor alpha 4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Package List Price ($)
$ 329