APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88118-PEP).
Product Note
This antibody is expected to recognize all reported isoforms: NP_944494.1; NP_000807.2; NP_944493.2. Note the aa numbers used in the name are from NP_944494.1.
Predict Reactivity
Human, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NNATHLQERDE, from the internal region of the protein sequence according to NP_944494.1; NP_000807.2; NP_944493.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gamma-aminobutyric acid type A receptor gamma2 subunit , CAE2 , ECA2 , GEFSP3
Cellular Localization
Cell membrane , Cell projection, dendrite
Background
This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88118 WB Image
WB analysis of mouse brain lysate using GTX88118 GABA A Receptor gamma 2 antibody, Internal. Dilution : 2μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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