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GABA B Receptor 1 antibody [N2C1], Internal

GTX102511_39897_20160520_IFA_19011506_766.jpg
GTX102511_39897_20160520_IFA_2_19011506_763.jpg
GTX102511_39897_WB_M_19011506_859.jpg
GTX102511_39897_IHC_2_19011506_500.jpg
GTX102511_39897_20160309_IHC-P_R_19011506_486.jpg
GTX102511_39897_WB_19011506_639.jpg

Cat No. GTX102511

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

PC-3 , U87-MG , SK-N-SH , C8D30

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GABA B Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gamma-Aminobutyric Acid Type B Receptor Subunit 1,Gababr1,Gabbr1-3,Gb1,Gprc3A,Gabbr1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Isoform 1E: Secreted

Background

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102511_39897_20160520_IFA_19011506_766.jpg

GTX102511 ICC/IF Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102511_39897_20160520_IFA_2_19011506_763.jpg

GTX102511 ICC/IF Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX102511_39897_WB_M_19011506_859.jpg

GTX102511 WB Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
7.5 % SDS-PAGE
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500

GTX102511_39897_IHC_2_19011506_500.jpg

GTX102511 IHC-P Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytosol on human brain by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102511_39897_20160309_IHC-P_R_19011506_486.jpg

GTX102511 IHC-P Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102511_39897_WB_19011506_639.jpg

GTX102511 WB Image

GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis.
A. 30 µg PC-3 whole cell lysate/extract
B. 30 µg U87-MG whole cell lysate/extract
C. 30 µg SK-N-SH whole cell lysate/extract
7.5 % SDS-PAGE
GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169