APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
PC-3 , U87-MG , SK-N-SH , C8D30
Predict Reactivity
Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human GABA B Receptor 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950325
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gamma-aminobutyric acid type B receptor subunit 1 , GABABR1 , GABBR1-3 , GB1 , GPRC3A
Cellular Localization
Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Isoform 1E: Secreted
Background
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102511 ICC/IF Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500. Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200. Blue: Hoechst 33342 staining.
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GTX102511 WB Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis. A. 30 μg C8D30 whole cell lysate/extract 7.5 % SDS-PAGE GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500
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GTX102511 IHC-P Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102511 IHC-P Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytosol on human brain by immunohistochemical analysis. Sample: Paraffin-embedded human brain. GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102511 WB Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABBR1 protein by Western blot analysis. A. 30 μg PC-3 whole cell lysate/extract B. 30 μg U87-MG whole cell lysate/extract C. 30 μg SK-N-SH whole cell lysate/extract 7.5 % SDS-PAGE GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) dilution: 1:500
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GTX102511 ICC/IF Image
GABA B Receptor 1 antibody [N2C1], Internal detects GABA B Receptor 1 protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABA B Receptor 1 protein stained by GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX102511 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 1 antibody [N2C1], Internal (GTX102511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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